منابع مشابه
Streptococcal Nucleases
Streptococcal DNAse C is more resistant to heat inactivation than the A or B enzyme. DNAses A and C are indifferent to the bacterial ribonucleic acid inhibitor whereas the B enzyme is markedly inhibited. Prolonged digestion with relatively large amounts of DNAse B results in chemical and biological destruction of the inhibitor. Ribonuclease as well as deoxyribonuclease activity is associated wi...
متن کاملSimple method for the preparation of streptococcal nucleases.
A simple method for preparation and fractionation of the streptococcal nucleases by polyacrylamide gel electrophoresis is presented. The procedure is carried out with ammonium sulfate-precipitated supernatant fluids from cultures of beta-hemolytic streptococci grown to stationary phase. Electrophoresis on polyacrylamide gel and subsequent elution of the fractionated enzymes allows the preparati...
متن کاملSingle-strand-specific nucleases.
Single-strand-specific nucleases, which act on single-stranded nucleic acids and single-stranded regions in double-stranded nucleic acids, are multifunctional enzymes and are ubiquitous in distribution. They find wide application as analytical tools in molecular biology research, although enzymes such as P1 nuclease are also used for production of flavor enhancers such as 5' IMP and 5' GMP. Bec...
متن کاملHuman mitochondrial nucleases
Mitochondria are cytosolic organelles that have many essential roles including ATP production via oxidative phosphorylation, apoptosis, iron-sulfur cluster biogenesis, heme and steroid synthesis, calcium homeostasis, and regulation of cellular redox state. One of the unique features of these organelles is the presence of an extrachromosomal mitochondrial genome (mtDNA), together with all the ma...
متن کاملGenome engineering with targetable nucleases.
Current technology enables the production of highly specific genome modifications with excellent efficiency and specificity. Key to this capability are targetable DNA cleavage reagents and cellular DNA repair pathways. The break made by these reagents can produce localized sequence changes through inaccurate nonhomologous end joining (NHEJ), often leading to gene inactivation. Alternatively, us...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1967
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.126.3.497